![]() ![]() If the disease cannot be contained, farmers may be required to destroy an entire field as a last resort. Once late blight is found in a farmer's field, that area must be destroyed along with its crops to prevent the threat of the disease spreading. Left untreated, the disease can cause widespread damage. ![]() The culprit was late blight disease - a fast spreading disease that causes mold symptoms on potato and tomato crops. The Plant Cell, 16(9), 2448-2462.Agriculture and Agri-Food potato famine in the 1840s was devastating to potato farmers and caused widespread hunger across the continent. (2004) Evidence for a direct link between glutathione biosynthesis and stress defense gene expression in Arabidopsis. (2014) Improving remote species identification through efficient training data collection. (2015) 2-cysteine peroxiredoxins and thylakoid ascorbate peroxidase create a water-water cycle that is essential to protect the photosynthetic apparatus under high light stress conditions. (2011) In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis. Current Opinion in Plant Biology, 7(4), 356-364.Īlbrecht, S.C., Barata, A.G., Großhans, J., Teleman, A.A. (2004) Strategies used by bacterial pathogens to suppress plant defenses. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.Ībramovitch, R.B. Phytophthora infestans chloroplast late blight potato redox imaging roGFP2 technical advance. The data suggest that mislocalization of chloroplast-targeted proteins is an efficient marker of late blight infection, and demonstrate how it can be utilized for non-destructive monitoring of the disease biotrophic stage using whole-plant redox imaging. Comparing redox with chlorophyll fluorescence imaging showed that infected leaf areas that exhibit mislocalized chl-roGFP2 also showed reduced non-photochemical quenching and enhanced quantum PSII yield (ΦPSII) compared with the surrounding leaf areas. Image analysis based on machine learning enabled systematic identification and quantification of spots, and unbiased classification of infected and uninfected leaves in inoculated plants. ![]() These spots were particularly evident during light-to-dark transitions, and reflected the mislocalization of chl-roGFP2 outside the chloroplasts. Clear spots on potato leaves with a lower chl-roGFP2 oxidation state were detected as early as 2 dpi, before any visual symptoms were recorded. infestans was detected by whole-plant redox imaging of potato plants expressing chloroplast-targeted reduction-oxidation sensitive green fluorescent protein (chl-roGFP2). Necrotic lesions are normally seen in leaves at 4 days post-inoculation (dpi) when colonized cells are dead, but early detection of the initial biotrophic growth stage, when the pathogen feeds on living cells, is challenging. Its early detection is crucial for suppressing disease spread. Late blight caused by the oomycete Phytophthora infestans is a most devastating disease of potatoes (Solanum tuberosum). ![]()
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